• Target：
• p21

• Fields：
• >>Endocrine resistance;>>Platinum drug resistance;>>ErbB signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Cell cycle;>>p53 signaling pathway;>>PI3K-Akt signaling pathway;>>Cellular senescence;>>JAK-STAT signaling pathway;>>Oxytocin signaling pathway;>>Parathyroid hormone synthesis, secretion and action;>>Cushing syndrome;>>Hepatitis C;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Epstein-Barr virus infection;>>Pathways in cancer;>>Transcriptional misregulation in cancer;>>Viral carcinogenesis;>>Proteoglycans in cancer;>>MicroRNAs in cancer;>>Colorectal cancer;>>Renal cell carcinoma;>>Pancreatic cancer;>>Endometrial cancer;>>Glioma;>>Prostate cancer;>>Thyroid cancer;>>Basal cell carcinoma;>>Melanoma;>>Bladder cancer;>>Chronic myeloid leukemia;>>Small cell lung cancer;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;

• Gene Name：
• CDKN1A

• Protein Name：
• Cyclin-dependent kinase inhibitor 1

• Human Gene Id：
• 1026

• Human Swiss Prot No：
• P38936

• Mouse Gene Id：
• 12575

• Mouse Swiss Prot No：
• P39689

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human p21 Cip1. AA range:111-160

• Specificity：
• p21 Polyclonal Antibody detects endogenous levels of p21 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CDKN1A;CAP20;CDKN1;CIP1;MDA6;PIC1;SDI1;WAF1;Cyclin-dependent kinase inhibitor 1;CDK-interacting protein 1;Melanoma differentiation-associated protein 6;MDA-6;p21;

• Observed Band(KD)：
• 21kD

• Background：
• This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lac

• Function：
• function:May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.,induction:By p53, mezerein (antileukemic compound) and interferon beta.,PTM:Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.,similarity:Belongs to the CDI family.,tissue specificity:Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.,

• Subcellular Location：
• Cytoplasm . Nucleus .

• Expression：
• Expressed in all adult tissues, with 5-fold lower levels observed in the brain.

Exosomal transfer of miR-181b-5p confers senescence-mediated doxorubicin resistance via modulating BCLAF1 in breast cancer BRITISH JOURNAL OF CANCER Zhao, Shaorong, Pan, Teng, Deng, Jinhai, Cao, Lixia, Vicencio, Jose M., Liu, Jingjing, Zhou, Guanglin, Ng, Tony, Zhang, Jin WB Human, Mouse Cal51 cell-xenograft, CALDOX cell-xenograft Cal51 cell, MCF-7 cell

Chidamide Combined With Doxorubicin Induced p53-Driven Cell Cycle Arrest and Cell Apoptosis Reverse Multidrug Resistance of Breast Cancer. Frontiers in Oncology Front Oncol. 2021 Mar;0:344 WB Human 1:1000 CALDOX cell，MCF-7 cell，A02 cell

Roles of eIF3m in the tumorigenesis of triple negative breast cancer. Cancer Cell International Cancer Cell Int. 2020 Dec;20(1):1-16 WB Human 1:500 MDA-MB-231 cell, MDA-MB-436,MCF-10A cell

Ginsenoside Rg1 improves fertility and reduces ovarian pathological damages in premature ovarian failure model of mice:. EXPERIMENTAL BIOLOGY AND MEDICINE Exp Biol Med. 2017;242(7):683-691 WB Mouse ovaries

Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:1000 Aortic

Suppression of CDCA3 inhibits prostate cancer progression via NF‑κB/cyclin D1 signaling inactivation and p21 accumulation. ONCOLOGY REPORTS Oncol Rep. 2022 Feb;47(2):1-10 WB Human 1:1000 Prostate cancer tissue,paracancerous tissue.

Sirtuin 7 promotes non‑small cell lung cancer progression by facilitating G1/S phase and epithelial‑mesenchymal transition and activating AKT and ERK1/2 signaling. ONCOLOGY REPORTS Oncol Rep. 2020 Sep;44(3):959-972 WB,IHC Human 1 : 1000,1:200 Lung cancer TMA section A549 cell, H292 cell

ZIC1 acts a tumor suppressor in breast cancer by targeting survivin. INTERNATIONAL JOURNAL OF ONCOLOGY Int J Oncol. 2018 Sep;53(3):937-948 WB Human 1:500 breast tumors MDA-MB-231 cell, SK-BR3 cell

Capsaicin inhibits proliferation and induces apoptosis in osteosarcoma cell lines via the mitogen‑activated protein kinase pathway. ONCOLOGY REPORTS 2017 Nov 01 WB Human MG63 cell,143B cell, HOS cell

Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:10000 Aortic

Chidamide Combined With Doxorubicin Induced p53-Driven Cell Cycle Arrest and Cell Apoptosis Reverse Multidrug Resistance of Breast Cancer. Frontiers in Oncology Front Oncol. 2021 Mar;0:344

Irisin ameliorates nicotine-mediated atherosclerosis via inhibition of the PI3K pathway. Annals of Translational Medicine Ann Transl Med. 2021 May; 9(9): 805 WB Mouse 1:10000 Aortic

Chidamide Combined With Doxorubicin Induced p53-Driven Cell Cycle Arrest and Cell Apoptosis Reverse Multidrug Resistance of Breast Cancer. Frontiers in Oncology Front Oncol. 2021 Mar;0:344

sST2: A Bridge Between Sirt1/p53/p21 Signal-Induced Senescence and TGF-β1/Smad2/3 Regulation of Cardiac Fibrosis in Mouse Viral Myocarditis INFLAMMATION Lu Hongxiang WB,IF Mouse Heart mouse cardiac fibroblasts (MCFs)

Identification of the Kupffer cell-derived circulating IGFBP-3 as a universal radiation biomarker for heavy ion, proton, and X-ray exposure. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Wenjun Wei WB Mouse 1:3000 mice Kupffer cell(MKC)

Protective effect of cornuside on OGD/R injury in SH-SY5Y cells and its underlying mechanism. BRAIN RESEARCH Jianwei Gong WB Human SH-SY5Y cell

L-Theanine delays D-galactose-induced senescence by regulating the cell cycle and inhibiting apoptosis in rat intestinal cells JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE Wen-Jun Xiao WB Rat 1:1000 IEC-6 cell

Paeonol Attenuates Atherosclerosis by Inhibiting Vascular Smooth Muscle Cells Senescence via SIRT1/P53/TRF2 Signaling Pathway MOLECULES Min Dai WB Mouse 1:2000 aorta tissue Vascular smooth muscle cell (VSMC)

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