• Target：
• p38

• Fields：
• >>Endocrine resistance;>>MAPK signaling pathway;>>Rap1 signaling pathway;>>FoxO signaling pathway;>>Sphingolipid signaling pathway;>>Oocyte meiosis;>>Cellular senescence;>>Adrenergic signaling in cardiomyocytes;>>VEGF signaling pathway;>>Osteoclast differentiation;>>Signaling pathways regulating pluripotency of stem cells;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>C-type lectin receptor signaling pathway;>>IL-17 signaling pathway;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>TNF signaling pathway;>>Leukocyte transendothelial migration;>>Thermogenesis;>>Neurotrophin signaling pathway;>>Retrograde endocannabinoid signaling;>>Dopaminergic synapse;>>Inflammatory mediator regulation of TRP channels;>>GnRH signaling pathway;>>Progesterone-mediated oocyte maturation;>

• Gene Name：
• MAPK14

• Protein Name：
• Mitogen-activated protein kinase 14

• Human Gene Id：
• 1432

• Human Swiss Prot No：
• Q16539

• Mouse Gene Id：
• 26416

• Mouse Swiss Prot No：
• P47811

• Rat Swiss Prot No：
• P70618

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human p38 MAPK. AA range:147-196

• Specificity：
• p38 Polyclonal Antibody detects endogenous levels of p38 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MAPK14;CSBP;CSBP1;CSBP2;CSPB1;MXI2;SAPK2A;Mitogen-activated protein kinase 14;MAP kinase 14;MAPK 14;Cytokine suppressive anti-inflammatory drug-binding protein;CSAID-binding protein;CSBP;MAP kinase MXI2;MAX-interacting protein

• Observed Band(KD)：
• 38kD

• Background：
• The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding d

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.,enzyme regulation:Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K3 or MAP2K6, and potentially also MAP2K4. Inhibited by dual specificity phosphatases, such as DUSP1. Specifically inhibited by the binding of pyridinyl-imidazole compounds, which are cytokine-suppressive anti-inflammatory drugs (CSAID). Isoform Mxi2 is 100-fold less sensitive to these agents than the other isoforms and is not inhibited by DUSP1. Isoform Exip is not activated by MAP2K6.,function:Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and seve

• Subcellular Location：
• Cytoplasm . Nucleus .

• Expression：
• Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.

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Proliferatins suppress lipopolysaccharide-induced inflammation via inhibition of the NF-κB and MAPK signaling pathways. BIOORGANIC CHEMISTRY2022 Jul;124:105810. Mouse RAW264.7 macrophages

Polydatin Improves Sepsis-Associated Encephalopathy by Activating Sirt1 and Reducing p38 Phosphorylation. JOURNAL OF SURGICAL RESEARCH2022 Aug;276:379. Mouse brain tissue

Therapeutic Potential of Synthetic Human β-Defensin 1 Short Motif Pep-B on Lipopolysaccharide-Stimulated Human Dental Pulp Stem Cells Mediat Inflamm. 2022;2022:6141967. WB Human 1 : 1000

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P38 MAPK/AKT signalling is involved in IL-33-mediated anti-apoptosis in childhood acute lymphoblastic leukaemia blast cells. ANNALS OF MEDICINE Ann Med. 2021;53(1):1464-1472 WB Human Bone marrow (BM) cell

Effect of cold stress on the MAPK pathway and lipidomics on muscle of Takifugu fasciatus. AQUACULTURE Aquaculture. 2021 Jul;540:736691 WB Fish 1:1500 Muscles

Interleukin-33 Promotes Cell Survival via p38 MAPK-Mediated Interleukin-6 Gene Expression and Release in Pediatric AML. Frontiers in Immunology Front Immunol. 2020; 11: 595053 WB Human bone marrow (BM)，peripheral blood (PB) HL-60 cell

Dibromoacetic Acid Induced Hepatotoxicity in Mice through Oxidative Stress and Toll-Like Receptor 4 Signaling Pathway Activation. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2019;2019:5637235 WB Mouse Liver

Novel paeonol derivatives: Design, synthesis and anti-inflammatory activity in vitro and in vivo. BIOORGANIC CHEMISTRY Bioorg Chem. 2020 May;98:103735 WB Mouse RAW264.7 cell

Anemonin Attenuates RANKL-Induced Osteoclastogenesis and Ameliorates LPS-Induced Inflammatory Bone Loss in Mice via Modulation of NFATc1. Frontiers in Pharmacology 2020 Feb 10 WB Mouse BMMs

Molecular Mechanisms of IRE1α-ASK1 Pathway Reactions to Unfolded Protein Response in DRN Neurons of Post-Traumatic Stress Disorder Rats. JOURNAL OF MOLECULAR NEUROSCIENCE J Mol Neurosci. 2017 Apr;61(4):531-541 WB Rat 1:500 brain

The Apolipoprotein C1 is involved in breast cancer progression via EMT and MAPK/JNK pathway. PATHOLOGY RESEARCH AND PRACTICE Pathol Res Pract. 2022 Jan;229:153746 WB Human Breast cancer tissue MDA-MB-231 cell,MCF-7 cell

Astragalus Polysaccharide Inhibits Ionizing Radiation-Induced Bystander Effects by Regulating MAPK/NF-κB Signaling Pathway in Bone Mesenchymal Stem Cells (BMSCs). MEDICAL SCIENCE MONITOR Med Sci Monitor. 2018; 24: 4649–4658 WB Human BMSCs

Structural characterization of a pectic polysaccharide from Codonopsis pilosula and its immunomodulatory activities in vivo and in vitro. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Int J Biol Macromol. 2017 Nov;104:1359 WB Mouse Splenocytes

β-Conglycinin-Induced Intestinal Porcine Epithelial Cell Damage via the Nuclear Factor κB/Mitogen-Activated Protein Kinase Signaling Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2019;67(32):9009–9021 WB Pig 1:800 IPEC-J2 cell

Soybean Glycinin- and β-Conglycinin-Induced Intestinal Damage in Piglets via the p38/JNK/NF-κB Signaling Pathway. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY J Agr Food Chem. 2018;66(36):9534–9541 WB,IHC Pig 1:500,1:1000 small intestine

Protective effects of flavonoids from the leaves of Carya cathayensis Sarg. against H₂O₂‑induced oxidative damage and apoptosis in vitro. Experimental and Therapeutic Medicine Exp Ther Med. 2021 Dec;22(6):1-11 WB Rat 1:1000 Rat aortic endothelial cells (RAECs)

Aβ40 Promotes the Osteoblastic Differentiation of Aortic Valve Interstitial Cells through the RAGE Pathway. Current Medical Science 2020 Oct 29 WB Human 1:1000 Acute aortic aortic valve interstitial cells (AVICs)

Peng, Chenglu, et al. "Glycinin-induced porcine IPEC-J2 cells damage via the NF-κB/MAPK signaling pathway." (2020).

TcpC Inhibits M1 but Promotes M2 Macrophage Polarization via Regulation of the MAPK/NF-κB and Akt/STAT6 Pathways in Urinary Tract Infection Cells Jianping Pan WB Human,Mouse

Ershiwuwei Lvxue Pill alleviates rheumatoid arthritis by different pathways and produces changes in the gut microbiota PHYTOMEDICINE Shaohui Wang WB Rat

Protective Effect of Lactiplantibacillus plantarum subsp. plantarum SC-5 on Dextran Sulfate Sodium—Induced Colitis in Mice Foods Pan Hu WB Mouse Colon tissue

ZFP36L1 Promotes Gastric Cancer Progression via Regulating JNK and p38 MAPK Signaling Pathways Recent Patents on Anti-Cancer Drug Discovery Fenghua Zhou WB Human SGC-7901, BGC-823 cell

Muscone attenuates susceptibility to ventricular arrhythmia by inhibiting NLRP3 inflammasome activation in rats after myocardial infarction. Yanhong Tang WB Rat 1:1000 hearts

Nickel induces blood-testis barrier damage through ROS-mediated p38 MAPK pathways in mice. Redox Biology Hongrui Guo WB Mouse testis tissue TM4 cell

Protective effect of cornuside on OGD/R injury in SH-SY5Y cells and its underlying mechanism. BRAIN RESEARCH Jianwei Gong WB Human SH-SY5Y cell

L-Theanine delays D-galactose-induced senescence by regulating the cell cycle and inhibiting apoptosis in rat intestinal cells JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE Wei Xu WB Rat 1:1000 IEC-6 cell

Design and synthesis of pterostilbene derivatives bearing triazole moiety that might treat DSS-induced colitis in mice through modulation of NF-κB/MAPK signaling pathways EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY Liuzeng Chen WB Mouse 1:1000 RAW264.7 cell

Study of the Mechanism of Astragali Radix in Treating Type 2 Diabetes Mellitus and Its Renal Protection Based on Enzyme Activity, Network Pharmacology, and Experimental Verification MOLECULES Chunnan Li WB Mouse renal tissue

Effect of the Pulsatilla decoction n-butanol extract on vulvovaginal candidiasis caused by Candida glabrata and on its virulence factors FITOTERAPIA Jiaping Zhang WB Mouse vaginal tissue

Design, synthesis, in vitro and in vivo biological evaluation of pterostilbene derivatives for anti-inflammation therapy JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY Liuzeng Chen WB Mouse 1:1000 RAW264.7 cell

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