Rab 35 Polyclonal Antibody
- Catalog No.:YT3931
- Applications:WB;ELISA;IHC
- Reactivity:Human;Mouse;Rat
- Target:
- Rab 35
- Fields:
- >>Endocytosis
- Gene Name:
- RAB35
- Protein Name:
- Ras-related protein Rab-35
- Human Gene Id:
- 11021
- Human Swiss Prot No:
- Q15286
- Mouse Gene Id:
- 77407
- Mouse Swiss Prot No:
- Q6PHN9
- Rat Gene Id:
- 288700
- Rat Swiss Prot No:
- Q5U316
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human RAB35. AA range:99-148
- Specificity:
- Rab 35 Polyclonal Antibody detects endogenous levels of Rab 35 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000;IHC 1:50-300; ELISA 2000-20000
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- RAB35;RAB1C;RAY;Ras-related protein Rab-35;GTP-binding protein RAY;Ras-related protein Rab-1C
- Observed Band(KD):
- 24kD
- Background:
- function:Possesses GTPase activity.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,
- Function:
- function:Possesses GTPase activity.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,
- Subcellular Location:
- Cell membrane ; Lipid-anchor ; Cytoplasmic side . Membrane, clathrin-coated pit . Cytoplasmic vesicle, clathrin-coated vesicle . Endosome . Melanosome . Present on sorting endosomes and recycling endosome tubules (PubMed:16950109). Tends to be enriched in PIP2-positive cell membrane domains (PubMed:16950109). During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis (PubMed:16950109). Identified in stage I to stage IV melanosomes (PubMed:17081065). .
- Expression:
- Brain,Muscle,Uterus,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using Rab 35 Polyclonal Antibody
- Western blot analysis of RAB35 Antibody. The lane on the right is blocked with the RAB35 peptide.
- Western blot analysis of the lysates from HT-29 cells using RAB35 antibody.
- Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).