Rabphilin-3A Polyclonal Antibody
- Catalog No.:YT3950
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- Rabphilin 3A
- Gene Name:
- RPH3A
- Protein Name:
- Rabphilin-3A
- Human Gene Id:
- 22895
- Human Swiss Prot No:
- Q9Y2J0
- Mouse Gene Id:
- 19894
- Mouse Swiss Prot No:
- P47708
- Rat Gene Id:
- 171039
- Rat Swiss Prot No:
- P47709
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human Rabphilin 3A. AA range:203-252
- Specificity:
- Rabphilin-3A Polyclonal Antibody detects endogenous levels of Rabphilin-3A protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- RPH3A;KIAA0985;Rabphilin-3A;Exophilin-1
- Observed Band(KD):
- 75kD
- Background:
- Exocytosis of neurotransmitters and hormones is fundamental to synaptic neurotransmission and cell-cell communication. RAB3A (MIM 179390) is a small G protein that is thought to act at late stages of exocytosis, and RPH3A is a RAB3A effector (Lin et al., 2007 [PubMed 17149709]).[supplied by OMIM, Jul 2008],
- Function:
- function:Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.,similarity:Contains 1 FYVE-type zinc finger.,similarity:Contains 1 RabBD (Rab-binding) domain.,similarity:Contains 2 C2 domains.,
- Subcellular Location:
- Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane . Cell projection, dendritic spine . Cell junction, synapse, postsynaptic cell membrane . Membrane ; Peripheral membrane protein .
- Expression:
- Brain,Lung,
- June 19-2018
- WESTERN IMMUNOBLOTTING PROTOCOL
- June 19-2018
- IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL
- June 19-2018
- IMMUNOFLUORESCENCE PROTOCOL
- September 08-2020
- FLOW-CYTOMEYRT-PROTOCOL
- May 20-2022
- Cell-Based ELISA│解您多样本WB检测之困扰
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN
- July 13-2018
- CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN
- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using Rabphilin-3A Polyclonal Antibody
- Immunofluorescence analysis of HeLa cells, using Rabphilin 3A Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Rabphilin 3A Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HeLa cells, treated with TNF-a 20ng/ml 2', using Rabphilin 3A Antibody. The lane on the right is blocked with the synthesized peptide.