• Target：
• Repac

• Fields：
• >>Ras signaling pathway;>>Rap1 signaling pathway

• Gene Name：
• RAPGEF5

• Protein Name：
• Rap guanine nucleotide exchange factor 5

• Human Gene Id：
• 9771

• Human Swiss Prot No：
• Q92565

• Mouse Gene Id：
• 217944

• Mouse Swiss Prot No：
• Q8C0Q9

• Rat Gene Id：
• 362799

• Rat Swiss Prot No：
• P83900

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human RAPGEF5. AA range:431-480

• Specificity：
• Repac Polyclonal Antibody detects endogenous levels of Repac protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000;IHC 1:50-300

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• RAPGEF5;GFR;KIAA0277;MRGEF;Rap guanine nucleotide exchange factor 5;Guanine nucleotide exchange factor for Rap1;M-Ras-regulated Rap GEF;MR-GEF;Related to Epac;Repac

• Observed Band(KD)：
• 60kD

• Background：
• Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008],

• Function：
• function:Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation.,similarity:Contains 1 N-terminal Ras-GEF domain.,similarity:Contains 1 Ras-GEF domain.,tissue specificity:Widely expressed with highest levels in brain.,

• Subcellular Location：
• Nucleus.

• Expression：
• Widely expressed with highest levels in brain.

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