Spindlin-1 Polyclonal Antibody
- Catalog No.:YT4385
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- Spindlin-1
- Gene Name:
- SPIN1
- Protein Name:
- Spindlin-1
- Human Gene Id:
- 10927
- Human Swiss Prot No:
- Q9Y657
- Mouse Gene Id:
- 20729
- Mouse Swiss Prot No:
- Q61142
- Rat Gene Id:
- 1.00364e+008
- Rat Swiss Prot No:
- Q4V8J7
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human SPIN1. AA range:111-160
- Specificity:
- Spindlin-1 Polyclonal Antibody detects endogenous levels of Spindlin-1 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- SPIN1;OCR;SPIN;Spindlin-1;Ovarian cancer-related protein
- Observed Band(KD):
- 30kD
- Background:
- function:May play a role in cell-cycle regulation during the transition from gamete to embryo.,PTM:Phosphorylated during oocyte meiotic maturation.,sequence caution:Translated as Glu.,similarity:Belongs to the SPIN/STSY family.,
- Function:
- function:May play a role in cell-cycle regulation during the transition from gamete to embryo.,PTM:Phosphorylated during oocyte meiotic maturation.,sequence caution:Translated as Glu.,similarity:Belongs to the SPIN/STSY family.,
- Subcellular Location:
- Nucleus . Nucleus, nucleolus .
- Expression:
- Highly expressed in ovarian cancer tissues.
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- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of Hela cell. 1,Spindlin-1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Bcl-2 Monoclonal Antibody(6B5)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
- Western Blot analysis of various cells using Spindlin-1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western Blot analysis of HeLa cells using Spindlin-1 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from HeLa cells, using SPIN1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from K562 cells using SPIN1 antibody.