• Target：
• TRAF6

• Fields：
• >>MAPK signaling pathway;>>NF-kappa B signaling pathway;>>Ubiquitin mediated proteolysis;>>Autophagy - animal;>>Endocytosis;>>Osteoclast differentiation;>>Toll-like receptor signaling pathway;>>NOD-like receptor signaling pathway;>>RIG-I-like receptor signaling pathway;>>IL-17 signaling pathway;>>Neurotrophin signaling pathway;>>Alcoholic liver disease;>>Pathogenic Escherichia coli infection;>>Shigellosis;>>Salmonella infection;>>Pertussis;>>Yersinia infection;>>Leishmaniasis;>>Chagas disease;>>Toxoplasmosis;>>Tuberculosis;>>Hepatitis C;>>Hepatitis B;>>Measles;>>Herpes simplex virus 1 infection;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>Coronavirus disease - COVID-19;>>Pathways in cancer;>>Small cell lung cancer;>>PD-L1 expression and PD-1 checkpoint pathway in cancer;>>Lipid and atherosclerosis

• Gene Name：
• TRAF6

• Protein Name：
• TNF receptor-associated factor 6

• Human Gene Id：
• 7189

• Human Swiss Prot No：
• Q9Y4K3

• Mouse Gene Id：
• 22034

• Mouse Swiss Prot No：
• P70196

• Rat Gene Id：
• 311245

• Rat Swiss Prot No：
• B5DF45

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human TRAF6. AA range:105-154

• Specificity：
• TRAF6 Polyclonal Antibody detects endogenous levels of TRAF6 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• TRAF6;RNF85;TNF receptor-associated factor 6;E3 ubiquitin-protein ligase TRAF6;Interleukin-1 signal transducer;RING finger protein 85

• Observed Band(KD)：
• 60kD

• Background：
• TNF receptor associated factor 6(TRAF6) Homo sapiens The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. This protein mediates signaling from members of the TNF receptor superfamily as well as the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has

• Function：
• domain:The coiled coil domain mediates homo- and hetero-oligomerization.,domain:The MATH/TRAF domain binds to receptor cytoplasmic domains.,function:Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Also involved in the IL-1 signaling pathway via MYD88 and IRAK kinases. Seems to be involved in IL-17 signaling (By similarity). Mediates activation of NF-kappa-B and JNK. May function as an E3 ubiquitin ligase.,pathway:Protein modification; protein ubiquitination.,PTM:Polyubiquitinated.,similarity:Contains 1 MATH domain.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 2 TRAF-type zinc fingers.,subunit:Homotrimer (Probable). Binds to TNFRSF5/CD40 and TNFRSF11A/RANK. Associates with NGFR, TNFRSF17, IRAK1, IRAK2, IRAK3, IRAK4, RIPK

• Subcellular Location：
• Cytoplasm . Cytoplasm, cell cortex . Nucleus . Lipid droplet . Found in the nuclei of some aggressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T- and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus. RSAD2/viperin recruits it to the lipid droplet (By similarity). .

• Expression：
• Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Gingival mesenchymal stem cell-derived exosomes are immunosuppressive in preventing collagen-induced arthritis. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE J Cell Mol Med. 2022 Feb;26(3):693-708 WB Mouse 1:1000 Paw

Dibromoacetic Acid Induced Hepatotoxicity in Mice through Oxidative Stress and Toll-Like Receptor 4 Signaling Pathway Activation. Oxidative Medicine and Cellular Longevity Oxid Med Cell Longev. 2019;2019:5637235 WB Mouse Liver

Sucralose Promotes Colitis-Associated Colorectal Cancer Risk in a Murine Model Along With Changes in Microbiota. Frontiers in Oncology Front Oncol. 2020 Jun;0:710 WB Mouse colon

The Functional Role of Fecal Microbiota Transplantation on Dextran Sulfate Sodium-Induced Colitis in Mice. Frontiers in Cellular and Infection Microbiology Front Cell Infect Mi. 2019 Nov;0:393 WB Mouse colon

CD137 Regulates NFATc1 Expression in Mouse VSMCs through TRAF6/NF-κB p65 Signaling Pathway. MEDIATORS OF INFLAMMATION Mediat Inflamm. 2015;2015:639780 WB Human 1 : 1000 VSMCs

Liu, Jun, et al. "Xylose rich heteroglycan from flaxseed gum mediates the immunostimulatory effects on macrophages via TLR2 activation." Carbohydrate polymers 213 (2019): 59-69.

Eif2s3y alleviated LPS-induced damage to mouse testis and maintained spermatogenesis by negatively regulating Adamts5. THERIOGENOLOGY Jinlian Hua WB Mouse 1:1000 testes

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