• Target：
• TRAP230

• Fields：
• >>Thyroid hormone signaling pathway

• Gene Name：
• MED12

• Protein Name：
• Mediator of RNA polymerase II transcription subunit 12

• Human Gene Id：
• 9968

• Human Swiss Prot No：
• Q93074

• Mouse Swiss Prot No：
• A2AGH6

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human MED12. AA range:611-660

• Specificity：
• TRAP230 Polyclonal Antibody detects endogenous levels of TRAP230 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500-2000 IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• MED12;ARC240;CAGH45;HOPA;KIAA0192;TNRC11;TRAP230;Mediator of RNA polymerase II transcription subunit 12;Activator-recruited cofactor 240 kDa component;ARC240;CAG repeat protein 45;Mediator complex subunit 12;OPA-containing prote

• Molecular Weight(Da)：
• 247kD

• Background：
• The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009],

• Function：
• disease:Defects in MED12 are the cause of Lujan-Fryns syndrome [MIM:309520]; also known as X-linked mental retardation with marfanoid habitus. Clinically, Lujan-Fryns syndrome can be distinguished from Opitz-Kaveggia syndrome by tall stature, hypernasal voice, hyperextensible digits and high nasal root.,disease:Defects in MED12 are the cause of Opitz-Kaveggia syndrome (OKS) [MIM:305450]; also known as FG syndrome type 1 (FGS1) or FG syndrome (FGS). OKS is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.,function:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters b

• Subcellular Location：
• Nucleus .

• Expression：
• Ubiquitous.

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