WDHD1 Polyclonal Antibody
- Catalog No.:YT4902
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- WDHD1
- Gene Name:
- WDHD1
- Protein Name:
- WD repeat and HMG-box DNA-binding protein 1
- Human Gene Id:
- 11169
- Human Swiss Prot No:
- O75717
- Mouse Swiss Prot No:
- P59328
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human WDHD1. AA range:721-770
- Specificity:
- WDHD1 Polyclonal Antibody detects endogenous levels of WDHD1 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- WDHD1;AND1;WD repeat and HMG-box DNA-binding protein 1;Acidic nucleoplasmic DNA-binding protein 1;And-1
- Observed Band(KD):
- 126kD
- Background:
- The protein encoded by this gene contains multiple N-terminal WD40 domains and a C-terminal high mobility group (HMG) box. WD40 domains are found in a variety of eukaryotic proteins and may function as adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
- Function:
- caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Binds DNA with high affinity. May also be involved in protein-protein interactions.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 HMG box DNA-binding domain.,similarity:Contains 7 WD repeats.,
- Subcellular Location:
- Nucleus, nucleoplasm .
- Expression:
- Embryonal rhabdomyosarcoma,Epithelium,Fetal brain,Liver,Ski
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using WDHD1 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western blot analysis of lysates from Jurkat and HepG2 cells, using WDHD1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).