α4Gn-T Polyclonal Antibody

    • Catalog No.:YT5000
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • α4Gn-T
      • Gene Name:
      • A4GNT
      • Protein Name:
      • Alpha-1,4-N-acetylglucosaminyltransferase
      • Human Swiss Prot No:
      • Q9UNA3
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human A4GNT. AA range:31-80
      • Specificity:
      • α4Gn-T Polyclonal Antibody detects endogenous levels of α4Gn-T protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • A4GNT;Alpha-1;4-N-acetylglucosaminyltransferase;Alpha4GnT
      • Observed Band(KD):
      • 39kD
      • Background:
      • This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. [provided by RefSeq, Jul 2008],
      • Function:
      • domain:The conserved DXD motif is involved in enzyme activity.,function:Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.,online information:Alpha-1,4-N-acetylglucosaminyltransferase,online information:GlycoGene database,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyltransferase 32 family.,tissue specificity:Detected in stomach and pancreas.,
      • Subcellular Location:
      • Golgi apparatus membrane ; Single-pass type II membrane protein .
      • Expression:
      • Detected in stomach and pancreas.
      • Products Images
      • Western blot analysis of lysates from K562 cells, using A4GNT Antibody. The lane on the right is blocked with the synthesized peptide.
      • Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).