α4Gn-T Polyclonal Antibody
- Catalog No.:YT5000
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- α4Gn-T
- Gene Name:
- A4GNT
- Protein Name:
- Alpha-1,4-N-acetylglucosaminyltransferase
- Human Gene Id:
- 51146
- Human Swiss Prot No:
- Q9UNA3
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human A4GNT. AA range:31-80
- Specificity:
- α4Gn-T Polyclonal Antibody detects endogenous levels of α4Gn-T protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- A4GNT;Alpha-1;4-N-acetylglucosaminyltransferase;Alpha4GnT
- Observed Band(KD):
- 39kD
- Background:
- This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. [provided by RefSeq, Jul 2008],
- Function:
- domain:The conserved DXD motif is involved in enzyme activity.,function:Necessary for the synthesis of type III mucin. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans.,online information:Alpha-1,4-N-acetylglucosaminyltransferase,online information:GlycoGene database,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyltransferase 32 family.,tissue specificity:Detected in stomach and pancreas.,
- Subcellular Location:
- Golgi apparatus membrane ; Single-pass type II membrane protein .
- Expression:
- Detected in stomach and pancreas.
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- Antibody-FAQs
- Products Images
- Western blot analysis of lysates from K562 cells, using A4GNT Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).