• Target：
• ENT1

• Fields：
• >>Alcoholism

• Gene Name：
• SLC29A1

• Protein Name：
• Equilibrative nucleoside transporter 1

• Human Gene Id：
• 2030

• Human Swiss Prot No：
• Q99808

• Mouse Gene Id：
• 63959

• Mouse Swiss Prot No：
• Q9JIM1

• Rat Gene Id：
• 63997

• Rat Swiss Prot No：
• O54698

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human ENT1. AA range:15-64

• Specificity：
• ENT1 Polyclonal Antibody detects endogenous levels of ENT1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• SLC29A1;ENT1;Equilibrative nucleoside transporter 1;Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter;Equilibrative NBMPR-sensitive nucleoside transporter;Nucleoside transporter, es-type;Solute carrier family 29 member 1

• Observed Band(KD)：
• 55kD

• Background：
• This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008],

• Function：
• function:Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).,PTM:Glycosylated.,similarity:Belongs to the SLC29A transporter family.,tissue specificity:Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.,

• Subcellular Location：
• Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Predominantly localized in the basolateral membrane in polarized MDCK cells.

• Expression：
• Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen.

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs