• Target：
• NCX1

• Fields：
• >>Calcium signaling pathway;>>cGMP-PKG signaling pathway;>>Cardiac muscle contraction;>>Adrenergic signaling in cardiomyocytes;>>Apelin signaling pathway;>>Olfactory transduction;>>Endocrine and other factor-regulated calcium reabsorption;>>Protein digestion and absorption;>>Mineral absorption;>>Hypertrophic cardiomyopathy;>>Arrhythmogenic right ventricular cardiomyopathy;>>Dilated cardiomyopathy

• Gene Name：
• SLC8A1

• Protein Name：
• Sodium/calcium exchanger 1

• Human Gene Id：
• 6546

• Human Swiss Prot No：
• P32418

• Mouse Gene Id：
• 20541

• Mouse Swiss Prot No：
• P70414

• Rat Gene Id：
• 29715

• Rat Swiss Prot No：
• Q01728

• Immunogen：
• Synthesized peptide derived from NCX1 . at AA range: 270-350

• Specificity：
• NCX1 Polyclonal Antibody detects endogenous levels of NCX1 protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• SLC8A1;CNC;NCX1;Sodium/calcium exchanger 1;Na(+)/Ca(2+)-exchange protein 1

• Observed Band(KD)：
• 108kD

• Background：
• In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma (plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.[supplied by OMIM, Apr 2004],

• Function：
• alternative products:Additional isoforms seem to exist,enzyme regulation:By ATP.,function:Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores.,similarity:Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily.,similarity:Contains 2 Calx-beta domains.,tissue specificity:Cardiac sarcolemma.,

• Subcellular Location：
• Cell membrane ; Multi-pass membrane protein .

• Expression：
• Detected primarily in heart and at lower levels in brain (PubMed:1374913). Expressed in cardiac sarcolemma, brain, kidney, liver, pancreas, skeletal muscle, placenta and lung (PubMed:1476165).

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