• Target：
• CIS

• Fields：
• >>JAK-STAT signaling pathway;>>Prolactin signaling pathway

• Gene Name：
• CISH G18

• Protein Name：
• Cytokine-inducible SH2-containing protein (CIS) (CIS-1) (Protein G18) (Suppressor of cytokine signaling) (SOCS)

• Human Gene Id：
• 1154

• Human Swiss Prot No：
• Q9NSE2

• Mouse Gene Id：
• 12700

• Mouse Swiss Prot No：
• Q62225

• Rat Swiss Prot No：
• O70512

• Immunogen：
• Synthetic peptide from human protein at AA range: 30-90

• Specificity：
• The antibody detects endogenous CIS

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IHC 1:50-200, ELISA 1:10000-20000. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• Cytokine-inducible SH2-containing protein (CIS;CIS-1;Protein G18;Suppressor of cytokine signaling;SOCS)

• Background：
• cytokine inducible SH2 containing protein(CISH) Homo sapiens The protein encoded by this gene contains a SH2 domain and a SOCS box domain. The protein thus belongs to the cytokine-induced STAT inhibitor (CIS), also known as suppressor of cytokine signaling (SOCS) or STAT-induced STAT inhibitor (SSI), protein family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by IL2, IL3, GM-CSF and EPO in hematopoietic cells. Proteasome-mediated degradation of this protein has been shown to be involved in the inactivation of the erythropoietin receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],

• Function：
• disease:CISH deletion may be involved in the pathogenesis of renal cell carcinomas and of lung cancer since lung and kidney tumors frequently exhibit 3p21.3 deletions.,function:SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.,induction:By a subset of cytokines including erythropoietin (EPO).,pathway:Protein modification; protein ubiquitination.,PTM:Association wi

• Subcellular Location：
• cytoplasm,cytosol,plasma membrane,

• Expression：
• Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.

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