Acetyl eIF5A/eIF5A2 (K47) Polyclonal Antibody
- Catalog No.:YK0021
- Applications:WB;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- eIF5A2
- Gene Name:
- EIF5A2
- Protein Name:
- Eukaryotic translation initiation factor 5A-2
- Human Gene Id:
- 56648
- Human Swiss Prot No:
- Q9GZV4
- Mouse Gene Id:
- 208691
- Mouse Swiss Prot No:
- Q8BGY2
- Immunogen:
- The antiserum was produced against synthesized Acetyl-peptide derived from human eIF5A around the Acetylation site of Lys47. AA range:11-60
- Specificity:
- Acetyl-eIF5A/eIF5A2 (K47) Polyclonal Antibody detects endogenous levels of eIF5A/eIF5A2 protein only when acetylated at K47.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- EIF5A2;Eukaryotic translation initiation factor 5A-2;eIF-5A-2;eIF-5A2;Eukaryotic initiation factor 5A isoform 2
- Molecular Weight(Da):
- 17kD
- Background:
- function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,tissue specificity:Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.,
- Function:
- function:The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.,PTM:eIF-5A seems to be the only eukaryotic protein to have an hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).,similarity:Belongs to the eIF-5A family.,tissue specificity:Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.,
- Subcellular Location:
- Cytoplasm . Nucleus . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Nucleus, nuclear pore complex . Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions. .
- Expression:
- Expressed in ovarian and colorectal cancer cell lines (at protein level). Highly expressed in testis. Overexpressed in some cancer cells.
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- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of MCF7 cell. 1,primary Antibody was diluted at 1:100(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody(catalog No: RS3611) was diluted at 1:500(room temperature, 50min).
- Western blot analysis of L929 lysis using antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Western blot analysis of lysate from L929 cells, using eIF5A (Acetyl-Lys47) Antibody.