• Target：
• ApoA-IV

• Fields：
• >>Fat digestion and absorption;>>Vitamin digestion and absorption;>>Cholesterol metabolism;>>Lipid and atherosclerosis

• Gene Name：
• APOA4

• Protein Name：
• Apolipoprotein A-IV

• Human Gene Id：
• 337

• Human Swiss Prot No：
• P06727

• Mouse Swiss Prot No：
• P06728

• Immunogen：
• Purified recombinant fragment of ApoA-IV (aa21-396) expressed in E. Coli.

• Specificity：
• ApoA-IV Monoclonal Antibody detects endogenous levels of ApoA-IV protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• APOA4;Apolipoprotein A-IV;Apo-AIV;ApoA-IV;Apolipoprotein A4

• Molecular Weight(Da)：
• 45kD

• References：
• 1. J Biol Chem. 2006 Feb 10;281(6):3560-8.
  2. Clin Chim Acta. 2008 Feb;388(1-2):78-83.

• Background：
• Apoliprotein (apo) A-IV gene contains 3 exons separated by two introns. A sequence polymorphism has been identified in the 3'UTR of the third exon. The primary translation product is a 396-residue preprotein which after proteolytic processing is secreted its primary site of synthesis, the intestine, in association with chylomicron particles. Although its precise function is not known, apo A-IV is a potent activator of lecithin-cholesterol acyltransferase in vitro. [provided by RefSeq, Jul 2008],

• Function：
• domain:Nine of the thirteen 22-amino acid tandem repeats (each 22-mer is actually a tandem array of two, A and B, related 11-mers) occurring in this sequence are predicted to be highly alpha-helical, and many of these helices are amphipathic. They may therefore serve as lipid-binding domains with lecithin:cholesterol acyltransferase (LCAT) activating abilities.,function:May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons.,online information:The Singapore human mutation and polymorphism database,polymorphism:Eight alleles have been characterized (APOA-IV*0 to APOA-IV*7). APOA-IV*1 is the major allele (90%), APOA-IV*2 is also common (8%), the others are rare alleles.,similarity:Belongs to the apolipoprotein A1/A4/E family.,tissue

• Subcellular Location：
• Secreted.

• Expression：
• Synthesized primarily in the intestine and secreted in plasma.

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