• Target：
• PAK2

• Fields：
• >>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Axon guidance;>>Focal adhesion;>>T cell receptor signaling pathway;>>Regulation of actin cytoskeleton;>>Pathogenic Escherichia coli infection;>>Human immunodeficiency virus 1 infection;>>Renal cell carcinoma

• Gene Name：
• PAK2

• Protein Name：
• Serine/threonine-protein kinase PAK 2

• Human Gene Id：
• 5062

• Human Swiss Prot No：
• Q13177

• Mouse Swiss Prot No：
• Q8CIN4

• Immunogen：
• Purified recombinant fragment of PAKγ expressed in E. Coli.

• Specificity：
• PAKγ Monoclonal Antibody detects endogenous levels of PAKγ protein.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Monoclonal, Mouse

• Dilution：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• Purification：
• Affinity purification

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• PAK2;Serine/threonine-protein kinase PAK 2;Gamma-PAK;PAK65;S6/H4 kinase;p21-activated kinase 2;PAK-2;p58

• Molecular Weight(Da)：
• 58kD

• References：
• 1. J Immunol. 2004 Jun 15;172(12):7324-34.
  2. J Mol Biol. 2007 Jul 20;370(4):620-32.

• Background：
• The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-402 and allows the kinase domain to adopt an active structure (By similarity). Following caspase cleavage, autophosphorylted PAK-2p34 is constitutively active.,function:The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated for

• Subcellular Location：
• [Serine/threonine-protein kinase PAK 2]: Cytoplasm. MYO18A mediates the cellular distribution of the PAK2-ARHGEF7-GIT1 complex to the inner surface of the cell membrane.; [PAK-2p34]: Nucleus. Cytoplasm, perinuclear region. Membrane; Lipid-anchor. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.

• Expression：
• Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.

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