CHD3(C-term) mouse mAb

    • Catalog No.：YM1249
    • Applications：WB;ICC;IP
    • Reactivity：Human;Mouse;Rat
      • Target：
      • CHD3
      • Gene Name：
      • chd3
      • Human Gene Id：
      • 1107
      • Human Swiss Prot No：
      • Q12873
      • Immunogen：
      • Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.
      • Specificity：
      • This antibody detects endogenous levels of CHD3 (C-terminus) and does not cross-react with related proteins.
      • Formulation：
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source：
      • Monoclonal, Mouse
      • Dilution：
      • wb 1:1000 icc 1:400
      • Purification：
      • The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
      • Concentration：
      • 1 mg/ml
      • Storage Stability：
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name：
      • 226;2600010P09Rik;AF020312;ATP dependent helicase CHD3;ATP-dependent helicase Chd3;CHD-3;Chd3;CHD3_HUMAN;Chd7;Chromodomain-helicase-DNA-binding protein 3;hZFH;MGC40857;Mi 2 autoantigen 240 kDa protein;Mi 2a;Mi-2 autoantigen 240 kDa protein;Mi2 ALPHA;Mi2-alpha;Prp7;Prp9 1;ZFH;Zinc finger helicase;zinc-finger helicase (Snf2-like).
      • Observed Band(KD)：
      • 260kD
      • Background：
      • This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
      • Function：
      • disease:One of the main antigens reacting with anti-MI-2 positive sera of dermatomyositis.,function:Probable transcription regulator.,sequence caution:Differs from position 1967 onward for unknown reasons.,similarity:Belongs to the SNF2/RAD54 helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 2 chromo domains.,similarity:Contains 2 PHD-type zinc fingers.,subunit:Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts via its C-terminal region with HABP4.,tissue specificity:Widely expressed.,
      • Subcellular Location：
      • Nucleus, PML body . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with centrosomes in interphase and mitosis. .
      • Expression：
      • Widely expressed.
      • Products Images
      • Western blot detection of CHD3 (C-terminus) in NTERA2,3T3,C6,K562 and Hela cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 260KDa.Observed band size: 260KDa.
      • Immunocytochemistry staining of HeLa cells fixed with -20℃ Methanol and using CHD3 (C-terminus) mouse mAb (dilution 1:400).
      • Immunoprecipitation analysis of Hela cell lysates using CHD3 mouse mAb.

      Attributes

      • Code：
      • YM1249
      • Size
      • 40μL / 100μL / 200μL
      • Price$：
      • 160 / 280 / 450
      • Delivery：
      • 2024-11-30
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