V5-Tag Monoclonal Antibody(3C8)
- Catalog No.：YM3005
- Applications：WB;IP;IF
- Reactivity：Species independent
- Target：
- V5-Tag
- Immunogen：
- Synthetic Peptide of V5-Tag
- Specificity：
- The antibody detects C-terminal, internal, and N-terminal V5-tag fusion proteins.
- Formulation：
- PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- Source：
- Monoclonal, Mouse
- Dilution：
- WB 1:5000 IP: 1:200 IF 1:1000
- Purification：
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- Storage Stability：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Background：
- The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. This V5-tag antibody is prepared by immunizing synthetic peptide GKPIPNPLLGLDST coupled to KLH.
PCBP2 maintains antiviral signaling homeostasis by regulating cGAS enzymatic activity via antagonizing its condensation Nat Commun. 2022 Mar;13(1):1-16. IF Human 1:1000
TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death & Disease Cell Death Dis. 2021 Jan;12(1):1-18 WB Mouse,Human 1:1000 Bel-7402 cell-xenograft Bel-7402 cell， SMMC-7721 cell
Ci antagonizes Hippo signaling in the somatic cells of the ovary to drive germline stem cell differentiation. CELL RESEARCH Cell Res. 2015 Sep;25(10):1152-1170 WB,IP Drosophila S2 cell
Chu, Hin, et al. "Targeting highly pathogenic coronavirus-induced apoptosis reduces viral pathogenesis and disease severity." Science Advances 7.25 (2021): eabf8577.
Altered host protease determinants for SARS-CoV-2 Omicron Science Advances Hin Chu WB Human 293T cell
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- Antibody-FAQs
- Products Images
- Altered host protease determinants for SARS-CoV-2 Omicron Science Advances Hin Chu WB Human 293T cell
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- Chu, Hin, et al. "Targeting highly pathogenic coronavirus-induced apoptosis reduces viral pathogenesis and disease severity." Science Advances 7.25 (2021): eabf8577.
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- Guo, S., Chen, Y., Yang, Y. et al. TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress. Cell Death Dis 12, 42 (2021).
- 1ug V5 fusion protein+ Primary antibody dilution at 1) 1:5000 2) 1:10000
- IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5)
- IP antibody use:5ug V5 Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with V5-tag fusion protein 3、IP(untransfted 293+anti-V5 mAb+Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 5、IP (transfected 293+anti-V5 mAb+ Protein G agarose) 6、IP (transfected 293+Protein G) 7、Recombinant protein (E.coli)
