PKD2 (phospho Ser876) Polyclonal Antibody

    • Catalog No.:YP0341
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • PKD2
      • Fields:
      • >>Rap1 signaling pathway;>>Aldosterone synthesis and secretion;>>Chemical carcinogenesis - reactive oxygen species
      • Gene Name:
      • PRKD2
      • Protein Name:
      • Serine/threonine-protein kinase D2
      • Human Swiss Prot No:
      • Q9BZL6
      • Mouse Swiss Prot No:
      • Q8BZ03
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human PKD2 around the phosphorylation site of Ser876. AA range:829-878
      • Specificity:
      • Phospho-PKD2 (S876) Polyclonal Antibody detects endogenous levels of PKD2 protein only when phosphorylated at S876.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • PRKD2;PKD2;HSPC187;Serine/threonine-protein kinase D2;nPKC-D2
      • Observed Band(KD):
      • 96kD
      • Background:
      • The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
      • Function:
      • catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by diacylglycerol and phorbol esters.,function:Calcium-independent, phospholipid-dependent, serine- and threonine-specific protein kinase.,PTM:Autophosphorylated. Phorbol esters stimulates autophosphorylation. Phosphorylation of Ser-876 correlates with the activation status of the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,tissue specificity:Widely expressed.,
      • Subcellular Location:
      • Cytoplasm . Cell membrane . Nucleus . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation. Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import. .
      • Expression:
      • Widely expressed.
      • Products Images
      • Western Blot analysis of various cells using Phospho-PKD2 (S876) Polyclonal Antibody diluted at 1:1000
      • Western Blot analysis of HELA cells using Phospho-PKD2 (S876) Polyclonal Antibody diluted at 1:1000
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKD2 (Phospho-Ser876) Antibody
      • Western blot analysis of lysates from NIH/3T3 cells treated with PMA 250ng/ml 15', using PKD2 (Phospho-Ser876) Antibody. The lane on the right is blocked with the phospho peptide.
      • Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).