CD5 (phospho Tyr453) Polyclonal Antibody

    • Catalog No.:YP0544
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • CD5
      • Fields:
      • >>Hematopoietic cell lineage
      • Gene Name:
      • CD5
      • Protein Name:
      • T-cell surface glycoprotein CD5
      • Human Gene Id:
      • 921
      • Human Swiss Prot No:
      • P06127
      • Mouse Swiss Prot No:
      • P13379
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human CD5 around the phosphorylation site of Tyr453. AA range:421-470
      • Specificity:
      • Phospho-CD5 (Y453) Polyclonal Antibody detects endogenous levels of CD5 protein only when phosphorylated at Y453.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • CD5;LEU1;T-cell surface glycoprotein CD5;Lymphocyte antigen T1/Leu-1;CD antigen CD5
      • Observed Band(KD):
      • 40kD
      • Background:
      • function:May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.,similarity:Contains 3 SRCR domains.,
      • Function:
      • function:May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.,similarity:Contains 3 SRCR domains.,
      • Subcellular Location:
      • Cell membrane; Single-pass type I membrane protein.
      • Expression:
      • Lymphocyte,Pancreas,Tonsil,
      • Products Images
      • Western Blot analysis of 293 cells using Phospho-CD5 (Y453) Polyclonal Antibody
      • Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD5 (Phospho-Tyr453) Antibody
      • Immunofluorescence analysis of HepG2 cells, using CD5 (Phospho-Tyr453) Antibody. The picture on the right is blocked with the phospho peptide.
      • Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30' , using CD5 (Phospho-Tyr453) Antibody. The lane on the right is blocked with the phospho peptide.