CD5 (phospho Tyr453) Polyclonal Antibody
- Catalog No.:YP0544
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat
- Target:
- CD5
- Fields:
- >>Hematopoietic cell lineage
- Gene Name:
- CD5
- Protein Name:
- T-cell surface glycoprotein CD5
- Human Gene Id:
- 921
- Human Swiss Prot No:
- P06127
- Mouse Gene Id:
- 12507
- Mouse Swiss Prot No:
- P13379
- Rat Gene Id:
- 54236
- Rat Swiss Prot No:
- P51882
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human CD5 around the phosphorylation site of Tyr453. AA range:421-470
- Specificity:
- Phospho-CD5 (Y453) Polyclonal Antibody detects endogenous levels of CD5 protein only when phosphorylated at Y453.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- CD5;LEU1;T-cell surface glycoprotein CD5;Lymphocyte antigen T1/Leu-1;CD antigen CD5
- Observed Band(KD):
- 40kD
- Background:
- function:May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.,similarity:Contains 3 SRCR domains.,
- Function:
- function:May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.,similarity:Contains 3 SRCR domains.,
- Subcellular Location:
- Cell membrane; Single-pass type I membrane protein.
- Expression:
- Lymphocyte,Pancreas,Tonsil,
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- July 13-2018
- Antibody-FAQs
- Products Images
- Western Blot analysis of 293 cells using Phospho-CD5 (Y453) Polyclonal Antibody
- Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD5 (Phospho-Tyr453) Antibody
- Immunofluorescence analysis of HepG2 cells, using CD5 (Phospho-Tyr453) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30' , using CD5 (Phospho-Tyr453) Antibody. The lane on the right is blocked with the phospho peptide.