• Target：
• VE-Cadherin

• Fields：
• >>Cell adhesion molecules;>>Leukocyte transendothelial migration;>>Fluid shear stress and atherosclerosis

• Gene Name：
• CDH5

• Protein Name：
• Cadherin-5

• Human Gene Id：
• 1003

• Human Swiss Prot No：
• P33151

• Mouse Gene Id：
• 12562

• Mouse Swiss Prot No：
• P55284

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human VE-Cadherin around the phosphorylation site of Tyr731. AA range:697-746

• Specificity：
• Phospho-VE-Cadherin (Y731) Polyclonal Antibody detects endogenous levels of VE-Cadherin protein only when phosphorylated at Y731.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• CDH5;Cadherin-5;7B4 antigen;Vascular endothelial cadherin;VE-cadherin;CD antigen CD144

• Observed Band(KD)：
• 130kD

• Background：
• This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015],

• Function：
• function:Cadherins are calcium dependent cell adhesion proteins.,function:Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.,similarity:Contains 5 cadherin domains.,subcellular location:Found at cell-cell boundaries and probably at cell-matrix boundaries.,tissue specificity:Endothelial tissues and brain.,

• Subcellular Location：
• Cell junction . Cell membrane ; Single-pass type I membrane protein . Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions. .

• Expression：
• Endothelial tissues and brain.

Tumor necrosis factor ligand-related molecule 1A maintains blood–retinal barrier via modulating SHP-1-Src-VE-cadherin signaling in diabetic retinopathy. FASEB JOURNAL Faseb J. 2021 Nov;35(11):e22008 WB Rat，Human 1:1000 HRMECs,RRMECs

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