• Target：
• PERK

• Fields：
• >>Mitophagy - animal;>>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Hepatitis C;>>Measles;>>Herpes simplex virus 1 infection;>>Lipid and atherosclerosis

• Gene Name：
• EIF2AK3

• Protein Name：
• Eukaryotic translation initiation factor 2-alpha kinase 3

• Human Gene Id：
• 9451

• Human Swiss Prot No：
• Q9NZJ5

• Mouse Swiss Prot No：
• Q9Z2B5

• Rat Gene Id：
• 29702

• Rat Swiss Prot No：
• Q9Z1Z1

• Immunogen：
• The antiserum was produced against synthesized peptide derived from human PEK/PERK around the phosphorylation site of Thr981. AA range:947-996

• Specificity：
• Phospho-PERK (T981) Polyclonal Antibody detects endogenous levels of PERK protein only when phosphorylated at T981.

• Formulation：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Source：
• Polyclonal, Rabbit,IgG

• Dilution：
• IF 1:50-200 WB 1:500-2000 ,IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.

• Purification：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration：
• 1 mg/ml

• Storage Stability：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• Other Name：
• EIF2AK3;PEK;PERK;Eukaryotic translation initiation factor 2-alpha kinase 3;PRKR-like endoplasmic reticulum kinase;Pancreatic eIF2-alpha kinase;HsPEK

• Observed Band(KD)：
• 130kD

• Background：
• The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015],

• Function：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) [MIM:226980]; also known as multiple epiphyseal dysplasia with early-onset diabetes mellitus. WRS is a rare autosomal recessive disorder, characterized by permanent neonatal or early infancy insulin-dependent diabetes and, at a later age, epiphyseal dysplasia, osteoporosis, growth retardation and other multisystem manifestations, such as hepatic and renal dysfunctions, mental retardation and cardiovascular abnormalities.,domain:The lumenal domain senses perturbations in protein folding in the ER, probably through reversible interaction with HSPA5/BIP.,enzyme regulation:Perturbation in protein folding in the endoplasmic reticulum (ER) promotes reversible dissociation from HSPA5/BIP and oligomerization, resulting in transautophosphorylation and kinase act

• Subcellular Location：
• Endoplasmic reticulum membrane; Single-pass type I membrane protein.

• Expression：
• Ubiquitous. A high level expression is seen in secretory tissues.

Valdecoxib Protects against Cell Apoptosis Induced by Endoplasmic Reticulum Stress via the Inhibition of PERK-ATF4-CHOP Pathway in Experimental Glaucoma

NLRP3 inflammasome and endoplasmic reticulum stress in the epileptogenic zone in temporal lobe epilepsy: molecular insights into their interdependence. NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY Neuropath Appl Neuro. 2020 Dec;46(7):770-785 WB,IF,IHC Mouse,Human 1:500，1:200，1:500 Temporal neocortices, bilateral hippocampi

Microcystin-LR ameliorates pulmonary fibrosis via modulating CD206 + M2-like macrophage polarization. Cell Death & Disease Cell Death Dis. 2020 Feb;11(2):1-15 WB Mouse,Rat pulmonary tissues RAW264.7 cell

Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress. American Journal of Cancer Research Am J Cancer Res. 2016; 6(5): 924–936 WB Human SGC-7901 cell, HGC cell

Endoplasmic Reticulum Stress in Spinal Cord Contributes to the Development of Morphine Tolerance. Frontiers in Molecular Neuroscience Front Mol Neurosci. 2018 Mar;11:72 IHC Rat 1:50 spinal dorsal horn

Citrus alkaline extracts prevent endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway. PHYTOMEDICINE Phytomedicine. 2021 Aug;89:153599 WB Human 1 : 1000 Lung A549 cell

Stress kinases, endoplasmic reticulum stress, and Alzheimer’s disease related markers in peripheral blood mononuclear cells from subjects with increased body weight. Scientific Reports 2016 Aug 02 WB Human PBMCs

PERK signalling pathway mediates single prolonged stress-induced dysfunction of medial prefrontal cortex neurons. APOPTOSIS Apoptosis. 2017 Jun;22(6):753-768 WB Rat 1:500 neurons

Limonin ameliorates dextran sulfate sodium-induced chronic colitis in mice by inhibiting PERK-ATF4-CHOP pathway of ER stress and NF-κB signaling. INTERNATIONAL IMMUNOPHARMACOLOGY Int Immunopharmacol. 2021 Jan;90:107161 IHC Mouse Colon

Differential Levels of Endoplasmic Reticulum Stress in Peripheral Blood Mononuclear Cells from Patients with Sudden Sensorineural Hearing Loss. MEDICAL SCIENCE MONITOR Med Sci Monitor. 2020; 26: e927328-1–e927328-8 WB Human 1:1000 Peripheral blood mononuclear cells (PBMCs)

Glucocorticoids protect HEI-OC1 cells from tunicamycin-induced cell damage via inhibiting endoplasmic reticulum stress. Open Life Sciences Open Life Sci. 2021 Jan;16(1):695-702 WB Mouse 1 : 1000 HEI-OC1 Cell

Chlamydia trachomatis plasmid-encoded protein pORF5 activates unfolded protein response to induce autophagy via MAPK/ERK signaling pathway. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2020 May 20 WB Human HeLa cell

The Regulatory Effect of VEGF-Ax on Rat Bone Marrow Mesenchymal Stem Cells' Angioblastic Differentiation and Its Proangiogenic Ability. STEM CELLS AND DEVELOPMENT Stem Cells Dev. 2020 May;29(10):667-677 WB Rat 1:3000 Bone marrow mesenchymal stem cells(BMMSCs )

Erythropoietin suppresses hepatic steatosis and obesity by inhibiting endoplasmic reticulum stress and upregulating fibroblast growth factor 21. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Int J Mol Med. 2019 Aug;44(2):469-478 WB Mouse 1:1000 Liver hepatocytes

Isorhamnetin protects against bleomycin-induced pulmonary fibrosis by inhibiting endoplasmic reticulum stress and epithelial-mesenchymal transition. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 2019 Jan 01 WB Human,Mouse 1:2000 lung A549 cell,HBECs

Effect of Gegen Qinlian Decoction on Hepatic Gluconeogenesis in ZDF Rats with Type 2 Diabetes Mellitus Based on the Farnesol X Receptor/Ceramide Signaling Pathway Regulating Mitochondrial Metabolism and Endoplasmic Reticulum Stress. Evidence-based Complementary and Alternative Medicine Evid-Based Compl Alt. 2021;2021:9922292 WB,IHC Rat Liver

Rce1 expression in renal cell carcinoma and its regulatory effect on 786-O cell apoptosis through endoplasmic reticulum stress. ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2017 Mar 01 WB Human 1:2000 renal cancer tissues 786-O cell

Cheng, Jinbo, et al. "Vitamin D combined with resveratrol prevents cognitive decline in SAMP8 mice." Current Alzheimer Research 14.8 (2017): 820-833.

Wang, Zhichao, et al. "Citrus alkaline extract prevents endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway." Phytomedicine (2021): 153599.

Cadmium induces liver dysfunction and ferroptosis through the endoplasmic stress-ferritinophagy axis ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Jiacheng Wu WB Mouse

Shikonin potentiates therapeutic efficacy of oxaliplatin through reactive oxygen species-mediated intrinsic apoptosis and endoplasmic reticulum stress in oxaliplatin-resistant colorectal cancer cells DRUG DEVELOPMENT RESEARCH Yajie Zhang WB Human,Mouse HCT116R-xenograft OXA-resistant CRC cells (HCT116R)

ROS Scavenging Nanozyme Modulates Immunosuppression for Sensitized Cancer Immunotherapy Advanced Healthcare Materials Hui Wei WB Mouse bone marrow-derived macrophages (BMDMs),Myeloid-derived suppressor cells (MDSCs)

Downregulation of RCN1 promotes pyroptosis in acute myeloid leukemia cells. Molecular Oncology Qiaoxia Zhang WB Human OCI/AML3 cell,NB4 cell

Apigenin-induced Apoptosis is Reduced via Endoplasmic Reticulum Stress and ATF6/PERK Signaling in Human Gastric Cancer Cells. Pharmacognosy Magazine Liying Hou WB Human SGC-7901 Cell

Maresin-1 Attenuates Sepsis-Associated Acute Kidney Injury via Suppressing Inflammation, Endoplasmic Reticulum Stress and Pyroptosis by Activating the AMPK/SIRT3 Pathway Journal of Inflammation Research Miaomiao Sun IHC,WB Mouse 1:200,1:1000 renal tissue

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