ARP-1 Polyclonal Antibody

    • Catalog No.:YT0335
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse;Rat
      • Target:
      • ARP-1
      • Gene Name:
      • NR2F2
      • Protein Name:
      • COUP transcription factor 2
      • Human Gene Id:
      • 7026
      • Human Swiss Prot No:
      • P24468
      • Mouse Swiss Prot No:
      • P43135
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human COT2. AA range:1-50
      • Specificity:
      • ARP-1 Polyclonal Antibody detects endogenous levels of ARP-1 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • NR2F2;ARP1;TFCOUP2;COUP transcription factor 2;COUP-TF2;Apolipoprotein A-I regulatory protein 1;ARP-1;COUP transcription factor II;COUP-TF II;Nuclear receptor subfamily 2 group F member 2
      • Observed Band(KD):
      • 45kD
      • Background:
      • This gene encodes a member of the steroid thyroid hormone superfamily of nuclear receptors. The encoded protein is a ligand inducible transcription factor that is involved in the regulation of many different genes. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010],
      • Function:
      • function:Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A.,similarity:Belongs to the nuclear hormone receptor family.,similarity:Belongs to the nuclear hormone receptor family. NR2 subfamily.,similarity:Contains 1 nuclear receptor DNA-binding domain.,subunit:Interacts with SQSTM1 (By similarity). Binds DNA as a homodimer.,tissue specificity:Found in all tissues.,
      • Subcellular Location:
      • Nucleus.
      • Expression:
      • Ubiquitous. Expressed in the stromal cells of developing fetal ovaries (PubMed:29478779).
      • Products Images
      • Western Blot analysis of various cells using ARP-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
      • Immunofluorescence analysis of HUVEC cells, using COT2 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from HUVEC cells, using COT2 Antibody. The lane on the right is blocked with the synthesized peptide.