Galectin-8 Polyclonal Antibody
- Catalog No.:YT1840
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- Galectin-8
- Gene Name:
- LGALS8
- Protein Name:
- Galectin-8
- Human Gene Id:
- 3964
- Human Swiss Prot No:
- O00214
- Mouse Gene Id:
- 56048
- Mouse Swiss Prot No:
- Q9JL15
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human LEG8. AA range:61-110
- Specificity:
- Galectin-8 Polyclonal Antibody detects endogenous levels of Galectin-8 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- LGALS8;Galectin-8;Gal-8;Po66 carbohydrate-binding protein;Po66-CBP;Prostate carcinoma tumor antigen 1;PCTA-1
- Observed Band(KD):
- 36kD
- Background:
- This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
- Function:
- domain:Contains two homologous but distinct carbohydrate-binding domains.,online information:Galectin-8 C-terminal CRD,online information:Galectin-8 long isoform,similarity:Contains 2 galectin domains.,tissue specificity:Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.,
- Subcellular Location:
- Cytoplasmic vesicle . Cytoplasm, cytosol .
- Expression:
- Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
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- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
- Western Blot analysis of 3T3 cells using Galectin-8 Polyclonal Antibody diluted at 1:1000
- Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using LEG8 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from NIH/3T3 cells, using LEG8 Antibody. The lane on the right is blocked with the synthesized peptide.