PKAα/β/γ Polyclonal Antibody
- Catalog No.:YT3749
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse;Rat;Pig
- Target:
- PKA
- Fields:
- >>Endocrine resistance;>>MAPK signaling pathway;>>Ras signaling pathway;>>Calcium signaling pathway;>>cAMP signaling pathway;>>Chemokine signaling pathway;>>Oocyte meiosis;>>Autophagy - animal;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Adrenergic signaling in cardiomyocytes;>>Vascular smooth muscle contraction;>>Wnt signaling pathway;>>Hedgehog signaling pathway;>>Apelin signaling pathway;>>Tight junction;>>Gap junction;>>Platelet activation;>>Circadian entrainment;>>Thermogenesis;>>Long-term potentiation;>>Retrograde endocannabinoid signaling;>>Glutamatergic synapse;>>Cholinergic synapse;>>Serotonergic synapse;>>GABAergic synapse;>>Dopaminergic synapse;>>Olfactory transduction;>>Taste transduction;>>Inflammatory mediator regulation of TRP channels;>>Insulin signaling pathway;>>Insulin secretion;>>GnRH signaling pathway;>>Ovarian steroidogenesis;>>Progesterone-mediated oocyte maturation;>>Estrogen signaling pathway;>>Melanogenesis;>>Thyroid hormo
- Gene Name:
- PRKACA/PRKACB
- Protein Name:
- cAMP-dependent protein kinase catalytic subunit alpha/beta
- Human Gene Id:
- 5566/5567
- Human Swiss Prot No:
- P17612/P22694/P22612
- Mouse Gene Id:
- 18747/18749
- Rat Gene Id:
- 293508
- Rat Swiss Prot No:
- P27791/P68182
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human PKA alpha/beta CAT. AA range:166-215
- Specificity:
- PKAα/β/γ cat Polyclonal Antibody detects endogenous levels of PKAα/β/γ cat protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- PRKACA;PKACA;cAMP-dependent protein kinase catalytic subunit alpha;PKA C-alpha;PRKACB;cAMP-dependent protein kinase catalytic subunit beta;PKA C-beta;PRKACG;cAMP-dependent protein kinase catalytic subunit gamma;PKA C-gamma
- Observed Band(KD):
- 40kD
- Background:
- This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Altern
- Function:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by cAMP.,function:Phosphorylates a large number of substrates in the cytoplasm and the nucleus.,PTM:Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,subcellular location:Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.,subunit:A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP ca
- Subcellular Location:
- Cytoplasm. Cell membrane. Nucleus . Mitochondrion . Membrane ; Lipid-anchor . Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in meiotically incompetent oocytes. Associated to mitochondrion as meiotic competence is acquired. Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity). Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (PubMed:21085490). .; [Isoform 2]: Cell projection, cilium, flagellum . Cytoplasmic vesicle, secretory vesicle, acrosome . Expressed in the midpiece region of the sperm flagellum (PubMed:10906071). Colocalizes with MROH2B and TCP11 on the acrosome and tail regions in round spermatids and spermatozoa regardle
- Expression:
- Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids.
Effects and related mechanism of alpha-adrenergic receptor inhibitor phentolamine in a rabbit model of acute pulmonary embolism combined with shock
Pituitary miRNAs target GHRHR splice variants to regulate GH synthesis by mediating different intracellular signalling pathways. RNA Biology Rna Biol. 2020;17(12):1754-1766 WB Pig,Rat primary pituitary cell,GH3 cell
Regulation of the firing activity by PKA‐PKC‐Src family kinases in cultured neurons of hypothalamic arcuate nucleus. JOURNAL OF NEUROSCIENCE RESEARCH 2019 Aug 12 WB Rat 1:500 ARC neuron
miR-709 inhibits GHRP6 induced GH synthesis by targeting PRKCA in pituitary. MOLECULAR AND CELLULAR ENDOCRINOLOGY Mol Cell Endocrinol. 2020 Apr;506:110763 WB Rat GH3 cell
Lipolysis and thermogenesis in adipose tissues as new potential mechanisms for metabolic benefits of dietary fiber. NUTRITION 2016 Jun 02 WB Mouse white adipose tissue (WAT) ,brown adipose tissue
Developmental Exposure to Atrazine Impairs Spatial Memory and Downregulates the Hippocampal D1 Dopamine Receptor and cAMP-Dependent Signaling Pathway in Rats. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Int J Mol Sci. 2018 Aug;19(8):2241 WB Rat 1:500 hippocampal tissue
Effects and Mechanisms of Sciadonic Acid on Colonic Transit Function through Regulating 5-HT4/cAMP/PKA/AQP4 Signaling Pathway in STC Model Mice JOURNAL OF NUTRITIONAL BIOCHEMISTRY Zhuoli Yu WB Mouse colon tissue
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- Antibody-FAQs
- Products Images
- Sun, Xiao‐Dong, et al. "Regulation of the firing activity by PKA‐PKC‐Src family kinases in cultured neurons of hypothalamic arcuate nucleus." Journal of neuroscience research 98.2 (2020): 384-403.
- Immunofluorescence analysis of Hela cell. 1,PKAα/β/γ cat Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
- Western blot analysis of lysates from 1) 22RV1, 2) Hela , 3) COLO205 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Tubulin β Monoclonal Antibody(5G3) (cat:YM3030) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour.
- Western Blot analysis of various cells using PKAα/β/γ cat Polyclonal Antibody diluted at 1:1000
- Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using PKA alpha/beta CAT Antibody. The picture on the right is blocked with the synthesized peptide.