TEF-4 Polyclonal Antibody
- Catalog No.:YT4597
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- TEF-4
- Fields:
- >>Hippo signaling pathway;>>Hippo signaling pathway - multiple species
- Gene Name:
- TEAD2
- Protein Name:
- Transcriptional enhancer factor TEF-4
- Human Gene Id:
- 8463
- Human Swiss Prot No:
- Q15562
- Mouse Gene Id:
- 21677
- Mouse Swiss Prot No:
- P48301
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human TEAD2. AA range:71-120
- Specificity:
- TEF-4 Polyclonal Antibody detects endogenous levels of TEF-4 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500-2000 IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- TEAD2;TEF4;Transcriptional enhancer factor TEF-4;TEA domain family member 2;TEAD-2
- Molecular Weight(Da):
- 49kD
- Background:
- function:Putative transcription factor that binds to the SPH and GT-IIC "enhansons" (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.,similarity:Contains 1 TEA DNA-binding domain.,
- Function:
- function:Putative transcription factor that binds to the SPH and GT-IIC "enhansons" (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.,similarity:Contains 1 TEA DNA-binding domain.,
- Subcellular Location:
- Nucleus.
- Expression:
- Brain,Eye,Skin,Testis,
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- Antibody-FAQs
- Products Images
- Immunofluorescence analysis of Hela cell. 1,TEF-4 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-actin Monoclonal Antibody(5B7)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
- Western Blot analysis of mouse-brain cells using TEF-4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunofluorescence analysis of HepG2 cells, using TEAD2 Antibody. The picture on the right is blocked with the synthesized peptide.
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TEAD2 Antibody. The picture on the right is blocked with the synthesized peptide.