VPS72 Polyclonal Antibody

    • Catalog No.:YT4891
    • Applications:WB;IHC;IF;ELISA
    • Reactivity:Human;Mouse
      • Target:
      • VPS72
      • Gene Name:
      • VPS72
      • Protein Name:
      • Vacuolar protein sorting-associated protein 72 homolog
      • Human Gene Id:
      • 6944
      • Human Swiss Prot No:
      • Q15906
      • Mouse Swiss Prot No:
      • Q62481
      • Immunogen:
      • The antiserum was produced against synthesized peptide derived from human VPS72. AA range:131-180
      • Specificity:
      • VPS72 Polyclonal Antibody detects endogenous levels of VPS72 protein.
      • Formulation:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • Source:
      • Polyclonal, Rabbit,IgG
      • Dilution:
      • WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
      • Purification:
      • The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
      • Concentration:
      • 1 mg/ml
      • Storage Stability:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • Other Name:
      • VPS72;TCFL1;YL1;Vacuolar protein sorting-associated protein 72 homolog;Protein YL-1;Transcription factor-like 1
      • Observed Band(KD):
      • 41kD
      • Background:
      • The protein encoded by this gene is a shared subunit of two multi-component complexes, the histone acetyltransferase complex TRRAP/TIP60 as well as the chromatin remodeling SRCAP-containing complex. The TRRAP/TIP60 complex acetylates nucleosomal histones important for transcriptional regulation, double strand DNA break repair and apoptosis. The SRCAP-containing complex catalyzes the exchange of histone H2A with the histone variant Htz1 (H2AFZ) into nucleosomes. This protein may be responsible for binding H2AFZ, which has a role in chromosome segregation. This protein may also have a role in regulating long-term hematopoietic stem cell activity. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012],
      • Function:
      • function:Could be a DNA-binding transcriptional regulator. May be involved in chromatin modification and remodeling.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the VPS72/YL1 family.,subunit:Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit HTATIP/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41 and VPS72/YL1. Component of a NuA4-related complex which contains EP400, TRRAP/PAF400, SRCAP, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, actin, ACTL6A/BAF53A, VPS72 and YEATS4/GAS41. Also part of a multiprotein complex which contains SRCAP and which binds to H2AFZ/H2AZ.,
      • Subcellular Location:
      • Nucleus .
      • Expression:
      • Fibroblast,Kidney,
      • Products Images
      • Western blot analysis of 293T lysis using VPS72 antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
      • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using VPS72 Antibody. The picture on the right is blocked with the synthesized peptide.
      • Western blot analysis of lysates from RAW264.7 and NIH/3T3 cells, using VPS72 Antibody. The lane on the right is blocked with the synthesized peptide.