SRp75 Polyclonal Antibody
- Catalog No.:YT4421
- Applications:WB;IHC;IF;ELISA
- Reactivity:Human;Mouse
- Target:
- SRp75
- Fields:
- >>Spliceosome;>>Herpes simplex virus 1 infection
- Gene Name:
- SRSF4
- Protein Name:
- Serine/arginine-rich splicing factor 4
- Human Gene Id:
- 6429
- Human Swiss Prot No:
- Q08170
- Mouse Swiss Prot No:
- Q8VE97
- Immunogen:
- The antiserum was produced against synthesized peptide derived from human SFRS4. AA range:111-160
- Specificity:
- SRp75 Polyclonal Antibody detects endogenous levels of SRp75 protein.
- Formulation:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- Source:
- Polyclonal, Rabbit,IgG
- Dilution:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- Purification:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration:
- 1 mg/ml
- Storage Stability:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- Other Name:
- SRSF4;SFRS4;SRP75;Serine/arginine-rich splicing factor 4;Pre-mRNA-splicing factor SRP75;SRP001LB;Splicing factor; arginine/serine-rich 4
- Observed Band(KD):
- 57kD
- Background:
- This gene encodes a member of the arginine/serine-rich splicing factor family. The encoded protein likely functions in mRNA processing. [provided by RefSeq, Feb 2009],
- Function:
- function:A probable role in alternative splice site selection during pre-mRNA splicing.,PTM:Extensively phosphorylated on serine residues in the RS domain.,similarity:Belongs to the splicing factor SR family.,similarity:Contains 2 RRM (RNA recognition motif) domains.,subunit:Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Interacts with PNN.,
- Subcellular Location:
- Nucleus speckle .
- Expression:
- Aorta endothelial cell,Cerebrum,Epithelium,Lymph,
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- Antibody-FAQs
- Products Images
- Western Blot analysis of various cells using SRp75 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemical analysis of paraffin-embedded Human thyroid gland. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from LOVO cells, using SFRS4 Antibody. The lane on the right is blocked with the synthesized peptide.